New PDF release: Chromatin and Chromosome Structure

By Hsueh Jei Li

ISBN-10: 0124505503

ISBN-13: 9780124505506

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Additional info for Chromatin and Chromosome Structure

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And Mura, C. (1975 chemistry 14, 1390. Reprinted with pe of the American Chemical Society. HSUEH JEI LI results shown in Figs. 9 and 10 clearly indicate preferential binding of histone H5 to C. perfringens DNA. The natural base sequence in chicken DNA is therefore less important than the A + Τ content in the competition for histone H5 binding. At least two different types of binding have to be considered when a ligand is complexed with a macromolecule: isolated binding, when the ligand binds to a site at which the neighboring sites are still unoccupied; and binding of the ligand to a site where an adjacent site is already occupied.

Tm and Tm are, the melting temperatures of free and protein-bound DNA base pairs. temperature of that transition, T m for the low melting band, and T m for the high melting band. The biphasic melting curve can be schematically described as in Fig. 2a (38). The free DNA segments in the complex The are denatured in the first phase of transition of T m . protein-bound DNA segments remain intact until the second phase of transition at T m . Since the hyperchromicity changes in both transitions A represented by the area under the melting band at T m ( T m ) A and T& ( T m ) ι are proportional to the faction of base pairs free or bound by the protein, the following equation can be used to calculate the fraction (F) of base pairs in the complex bound by the protein (30): F = -35_ 2S ARM *v + (2) \ A where At is the total melting area which is equal to h m a x.

2, 1525 (1975). Finch, J. , and Kornberg, R. D . , Proc. Nat. Acad. Sei. A. 72, 3320 (1975). Gorovsky, Μ. , and Keevert, J. , Proc. Nat. Acad. Sei. A. 72, 3536 (1975). Pardon, J. , Worcester, D . , Wooley, J. , Van Holde, Κ. Ε . , and Richards, Β. , Nucl. Acids Res. 2, 2163 (1975). Simpson, R. , and Whitlock, J. , Nucl. Acids Res. 3, 117 (1976). Varshavsky, A. , Bakayev, V. , and Georgiev, G. , Nucl. Acids Res. 3, All (1976). Shaw, B. , Herman, T. , Kovacic, R. , Beaudreau, G. , and Van Holde, Κ.

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Chromatin and Chromosome Structure by Hsueh Jei Li

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