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Additional info for Erzeugung von Krankheitszuständen durch das Experiment: Kohlenhydratstoffwechsel, Fieber / Carbohydrate Metabolism, Fever

Sample text

Repeated injections of glucagon cause glycogenolysis, hyperglycemia, depletion of hepatic glycogen, negative nitrogen balance and fat mobilization (222, 223, 330, 395, 450, 452, 721). These effects are similar to those occurring in fasting and in diabetes and suggest that, under proper experimental conditions, glucagonmay be a diabetogenic agent. The first experiments designed to produce glucagon diabetes were only partially 8uccessful: repeated injections of semi-purified glucagon preparations decreased glucose tolerance in animals receiving cortisone, ACTH or growth hormone, caused an exacerbation of glycosuria in partially depancreatized force-fed rats and a transitory hyperglycemia in normal guinea pigs.

In these tests erythrocytes are conjugated with insulin by means of tannic acid or bis-diazobenzidine and either agglutinated by anti-insulin sera or used in a complement fixation system (40, 42, 44, 521, 824, 1017, 1349, 1355, 1481). I) Interferenee with allergie response to insulin in sensitized skin (898). g) Interferenee with insulin degradation (1561). h) Inhibition 01 enzyme aetivity with or without reversal by insulin (171, 242, 713, 804, 1491). 7. The use 01 insulin antisera lor the produetion 01 hyperglyeemia and 01 experimental diabetes in the intaet animal The passive transfer of serum from an immunized animal may cause acute insulin insufficiency in an animal of the same or of a different species (37-39,550, 750, 782, 1018, 1021, 1033, 1175, 1292, 1388, 1558, 1578).

After 6 to 8 months of treatment some of the animals became ill and were found to be diabetic. At about the same time HOUSSAY and his collaborators (683-686) succeeded in producing diabetes in normal and partially depancreatized animals by a few daily injections of crude anterior pituitary extracts. These results were confirmed in normal and partially depancreatized dogs, cats, rats and ferrets, in the adrenalectomized dog, in mice with the hereditary obese hyperglycemic syndrome and in the chick embryo (157, 695, 768, 881, 889, 906, 907, 955, 1308).

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