By H. E. Meyer, R. Kellner, F. Lottspeich
The papers gathered during this quantity signify the lawsuits of a workshop at the microcharacterization of proteins held in 1994. The authors and members to this article are affiliated with best laboratories, between them the ecu Molecular Biology Laboratory (EMBL) at Heidelberg and the Max Planck Institute for Biochemistry at Martinsried. a number of tools within the structural research of proteins are certain, together with these utilizing very small quantities of pattern.
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The papers accrued during this quantity signify the court cases of a workshop at the microcharacterization of proteins held in 1994. The authors and members to this article are affiliated with top laboratories, between them the ecu Molecular Biology Laboratory (EMBL) at Heidelberg and the Max Planck Institute for Biochemistry at Martinsried.
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Chem. 262,785-794. Deamidation, isomerization, and racemization at asparaginyl and aspartyl residues in peptides. S. (1975) Chemiccrl modification ofyroteins. North-Holland, Amsterdam. , Witkop, B. Soc. 83,1510-1511. Selective cleavage of the methionyl peptide bonds in ribonuclease with cyanogen bromide. S. Enzymo1. 91,324-332. Cleavage at Aspartic acid. , Edman, P. (1970) AnaLBiochem. 37,73430. Mechanism of cyanogen bromide reaction with methionine in peptide and proteins. C. Chem. 248,6583-6591.
The illustration was made available by Dr. P. Chervet, LC Packings. 0 10 20 30 43 40 Time (min) supplemental material, 1994, kindly provided by Dr. P. Chervet]. D. x 30 cm, C18, LC Packings) and separated in a solvent system containing TFNacetonitrile. The flow rate was 180 nYmin. Detection was performed at 214 nm with a Z-shaped flow cell (9 nl, LC Packings). 44 M. E. W. (1989) J . High Resolution Chromatography 12(5),278-28 1, Ultra-sensitive UV detection in micro separation. P. (1992) LCGC 10(2),140-148.
1989) New hydrophilic polymer for protein separations by HPLC. 348-356, Academic Press, San Diego. S. (1991a) Mobile phase preparation and column maintenance. 37-45, CRC Press, Boca Raton. S. (1991b) Mobile phase preparation and column maintenance. 69-94, CRC Press, Boca Raton. S. (1991~)The effects of anionic ion-pairing reagents on peptide retention in reversed-phase chromatography. 327-341, CRC Press, Boca Raton. W. Chem. Hoppe-Seyler 368,1607- 1611. Npmethylhistidine in myosin-light-chain kinase.
Microcharacterization of Proteins by H. E. Meyer, R. Kellner, F. Lottspeich